Examination of ribosome-like particles in isolated prolamellar bodies
- 1 January 1977
- journal article
- research article
- Published by Springer Nature in Planta
- Vol. 134 (1), 45-52
- https://doi.org/10.1007/bf00390093
Abstract
Potential methods for the preparation of fractions enriched in prolamellar bodies (PLBs) were examined in detail. Sucrose density gradient centrifugation methods gave fractions consisting almost exclusively of PLBs whilst those methods employing differential centrifugation were quite successful but contained greater quantities of lamellar membranes. Greater difficulty was experienced in obtaining detached PLBs which retained their “ribosome-like” lattice particles. No modification to density gradient procedures was found which retained these particles but the omission of ethylene diaminetetraacetic acid (EDTA) from all media including that of lysis gave a hint that this was possible with differential centrifugal methods. This was developed to produce a successful method for the preparation of PLBs which retain the “ribosome-like” particles of the lattice. Such fractions from Avena sativa L. and Hordeum vulgare L. were treated with ribonuclease which completely removed these particles from the lattice structures implying that they may be “ribosomal” in nature. EDTA apparently has a critical effect on PLB structure at concentration lower than those that effect the chloroplast coupling factor particles but it is not known if it is a direct effort of PLB membranes, on the lattice particles or both.This publication has 46 references indexed in Scilit:
- Protochlorophyll and protochlorophyllide as precursors for chlorophyll synthesis in vitroFEBS Letters, 1974
- Source of reducing equivalents for the in vitro synthesis of chlorophyll from protochlorophyllFEBS Letters, 1974
- Ultrastructural changes in isolated plastidsProtoplasma, 1973
- Chloroplast Development: Fine Structure and Chlorophyll SynthesisThe Quarterly Review of Biology, 1972
- Effect of chloramphenicol on membrane transformations in plastidsCanadian Journal of Botany, 1971
- Energy transfer between protochlorophyllide molecules: Evidence for multiple chromophores in the photoactive protochlorophyllide-protein complex in vivo and in vitroJournal of Molecular Biology, 1970
- ROUGH THYLAKOIDS: POLYSOMES ATTACHED TO CHLOROPLAST MEMBRANESThe Journal of cell biology, 1969
- A low-viscosity epoxy resin embedding medium for electron microscopyJournal of Ultrastructure Research, 1969
- The greening process in plastidsProtoplasma, 1965
- Über Membranbildungsprozesse im ChloroplastenPlanta, 1963