A Micro Method for Performing Parainfluenza Virus Neutralization Tests.

Abstract

Summary A micro technic has been devised for performing neutralization tests with the human and animal strains of parainflu-enza viruses. Tests are conducted in a rhesus monkey kidney cell system in disposable microplates; virus is detected by the hemad-sorption technic and results are read with a standard light microscope. The microneutrali-zation technic was shown to be a highly reliable method for demonstrating significant neutralizing antibody titer rises with sera from patients from whom parainfluenza viruses were isolated and also for detecting antibody to the animal strains in the sera of laboratory animals. The test may also be used for identification of viral isolates, but this application is limited by the fact that certain isolates do not possess sufficiently high titers to produce clear-cut hemadsorption in the micro system.