Liposome‐mediated labeling of adrenocorticotropin fragments parallels their biological activity
Open Access
- 11 July 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 158 (1), 12-16
- https://doi.org/10.1016/0014-5793(83)80666-8
Abstract
To test our hypothesis that specific interactions of ACTH peptides with model lipid membranes reflect the biological importance of similar interactions on target cells, we investigated the liposome‐mediated labeling of ACTH fragments with the extremely hydrophobic photolabel, 3‐trifluoromethyl‐3‐(m‐[125I]iodophenyl)diazirine. Correlations were found between the labeling rates and the agonistic and antagonistic potencies of the peptides for in vitro steroidogenesis and inhibition of a synaptosomal protein kinase. A model for the cross‐reactivity between ACTH and opioid peptides is discussed.Keywords
This publication has 19 references indexed in Scilit:
- The multiple site β-endorphin receptorTrends in Pharmacological Sciences, 1983
- A molecular basis for the interaction of corticotropin with opiate receptorsFEBS Letters, 1982
- Selective labeling of the hydrophobic core of membranes with 3-(trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine, a carbene-generating reagentBiochemistry, 1981
- Solution conformations of the pituitary opioid peptide dynorphin-(1–13)Biochemical and Biophysical Research Communications, 1981
- The adsorption of adrenocorticotropin-(1-24)-tetracosapeptide to lecithin bilayer membranes formed from liposomesEuropean Biophysics Journal, 1981
- Evidence for Homologous Actions of Pro-Opiocortin ProductsScience, 1980
- The Role of Membrane Lipids in Receptor MechanismsAnnual Review of Pharmacology and Toxicology, 1980
- ADRENOCORTICOTROPHIC HORMONE FRAGMENTS MIMIC THE EFFECT OF MORPHINE in vitroBritish Journal of Pharmacology, 1978
- ACTH: A SHORT INTRODUCTORY REVIEW*Annals of the New York Academy of Sciences, 1977
- Stereospecific morphine adsorption to phosphatidyl serine and other membranous components of brainEuropean Journal of Pharmacology, 1975