INCORPORATION OF THYMIDINE-H-3 AND C-14-LABELED AMINO ACIDS INTO VENTRAL PROSTATE AFTER INVIVO TREATMENT WITH ESTRADIOL-3N-BIS(2-CHLOROETHYL)CARBAMATE-17-BETA-PHOSPHATE (ESTRACYT) AND ITS ESTROGEN AND CYTOSTATIC PARTS

Abstract

The in vivo effect of Estracyt (a nitrogen mustard linked to estradiol-17.beta.-phosphate) on testosterone stimulated incorporation of 3H-thymidine and 14C-amino acids into the ventral prostate of castrated rats was investigated. A dose of 100 mg of Estracyt/kg of body weight injected i.v. daily for 3 days significantly reduced isotope incorporation. Equivalent amounts of the 2 main components of Estracyt, the nitrogen mustard and estradiol-17.beta.-phosphate, were not able to mimic this Estracyt effect. Possible implications of this difference and the intracellular antitumor mechanism of action of Estracyt are discussed.