Factors important in the laboratory production of relatively large amts. of Brucella suis cells were investigated. Techniques for the determination of light transmittance, catalase activity, pH, oxidation-reduction potential, and plate count were surveyed and modified. Statistical analyses were made of important assays. Growth curves were established for shaker cultures in fortified tryptose broth, using concurrent evaluations of the foregoing assays. Differences in the amount of inocula manifested themselves primarily in the length of the growth period, but the rate and extent of growth remained virtually unaffected. Differences in the age of the culture from which inocula were prepd. apparently were of no significant effect in these respects.