Essential Growth Factor in Serum Dialysate for Chick Skeletal Muscle Fibroblasts.

Abstract

Summary Chick skeletal muscle fibroblasts in monolayer cultures grow actively in homologous undialyzed serum supplemented with Medium 199. Dialysis of chicken serum removes a factor essential for growth and maintenance of these cells. The deficiency syndrome in cultures with dialyzed serum can be corrected by chicken serum dialysate, but not by inositol, cholesterol, vit. B₁₂, spleen nucleoprotein fraction, or by a more complex synthetic nutrient, NCTC 109. Proteosepeptone at 0.1% may be substituted for serum dialysate to restore full activity of whole serum.