The Effect Of Temperature and Of Relative Humidity On Sectioning Of Tissues Embedded In Polyethylene Glycol Wax

Abstract

Experiments carried out in a sealed room, where temperature and humidity could be controlled at will, showed that a high relative humidity in the atmosphere interferes with microtome cutting of tissues embedded in polyethylene glycol wax. The higher the atmospheric temperature the less became the degree of relative humidity which could be tolerated if successful sections were to be obtained.