A Rapid, High-Yield Method of Producing M 0-[12]a14Iodoinsujlin
- 1 October 1984
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 14 (4), 303-311
- https://doi.org/10.1080/10826068408070637
Abstract
We have developed a rapid method for producing homogeneous mono-[12]A14 iodoinsulin with high specific activity and yield. After iodination by the lactoperoxidase method, the labeled peptides were applied to a C18 PorasilR prs-column, washed with aqueous buffer to eliminate the free [12]-iodide and placed “in-line” with a C-18 HPLC column; mono-[12]A14 iodoinsulin was then eluted isocratically with 29% aceto-nitrile in 16 minutes. The labeled hormone was extremely stable, and proved suitable for various biological studies.This publication has 4 references indexed in Scilit:
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- HOMOGENEOUS MONO-I-125-LABELED INSULINS - PREPARATION AND CHARACTERIZATION OF MONO-I-125-LABELED (TYR A14)-INSULIN AND MONO-I-125-LABELED (TYR A19)-INSULIN1980
- Biological potency and binding affinity of monoiodoinsulin with iodine in tyrosine A14 or tyrosine A19Biochemical and Biophysical Research Communications, 1979
- Monoiodoinsulin: Demonstration of its biological activity and binding to fat cells and liver membranesBiochemical and Biophysical Research Communications, 1971