THE INSULIN ACTIVITY OF BOVINE AND OVINE BLOOD PLASMA
- 1 September 1962
- journal article
- research article
- Published by Bioscientifica in Journal of Endocrinology
- Vol. 25 (1), 35-42
- https://doi.org/10.1677/joe.0.0250035
Abstract
SUMMARY: A standardized method for the assay of small amounts of insulin (10–1000 μu./ml.) by measuring the effect on the glucose uptake of rat diaphragm in vitro is described. The most sensitive part of the concentration/response curve is within the range 10–150 μu./ml., and over this range there is a straight-line relationship between insulin concentration and insulin effect. The importance of having a protein such as gelatin present in dilute solutions of insulin is discussed.Keywords
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