Transcriptome and Proteome Analysis ofBacillus subtilisGene Expression Modulated by Amino Acid Availability
Open Access
- 1 August 2002
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 184 (15), 4288-4295
- https://doi.org/10.1128/jb.184.15.4288-4295.2002
Abstract
A comprehensive study of Bacillus subtilis gene expression patterns in response to amino acid availability was performed by means of proteomics and transcriptomics. The methods of two-dimensional protein gel electrophoresis and DNA macroarray technology were combined to analyze cells exponentially grown in minimal medium with and without 0.2% Casamino Acids (CAA). This approach revealed about 120 genes predominantly involved in amino acid biosynthesis, sporulation, and competence, which were downregulated in CAA-containing medium. Determination of sporulation frequencies confirmed the physiological relevance of the expression data.Keywords
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