Thetin–homocysteine transmethylase. A preliminary manometric study of the enzyme from rat liver
- 1 May 1956
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 63 (1), 116-124
- https://doi.org/10.1042/bj0630116
Abstract
The methylation of homocysteine by dimethylthetin in the presence of rat-liver suspensions was studied by estimation of the methionine formed. The estimation of methionine under the particular experi-mental conditions employed is discussed. A manometric method for following the course of the transmethylation, based on the formation of acid in the reaction, is described. The enzyme involved, thetin-homo-cysteine transmethylase, was partially purified from rat liver by am-monium sulfate fractionation and some of its properties were investi-gated.Keywords
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