Determination of molecular weights by fluctuation spectroscopy: application to DNA.
- 1 August 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (8), 2776-2780
- https://doi.org/10.1073/pnas.73.8.2776
Abstract
A method for determining MW of macromolecules by measuring spontaneous concentration fluctuations is described. The method is absolute, rapid and requires no shearing forces on the molecules. This technique was applied to the determination of MW of DNA molecules. The MW values obtained for T2 phage DNA (1.14 .times. 108) and replicating Escherichia coli DNA (3.9 .times. 109) agree with previous results. By monitoring individual molecules, an estimate of the MW of nuclei and individual chromosomal DNA molecules of Drosophila melanogaster was obtained.This publication has 19 references indexed in Scilit:
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