Determination of molecular weights by fluctuation spectroscopy: application to DNA.
- 1 August 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (8), 2776-2780
- https://doi.org/10.1073/pnas.73.8.2776
Abstract
A method for determining MW of macromolecules by measuring spontaneous concentration fluctuations is described. The method is absolute, rapid and requires no shearing forces on the molecules. This technique was applied to the determination of MW of DNA molecules. The MW values obtained for T2 phage DNA (1.14 .times. 108) and replicating Escherichia coli DNA (3.9 .times. 109) agree with previous results. By monitoring individual molecules, an estimate of the MW of nuclei and individual chromosomal DNA molecules of Drosophila melanogaster was obtained.This publication has 19 references indexed in Scilit:
- DNA polyintercalating drugs: DNA binding of diacridine derivatives.Proceedings of the National Academy of Sciences, 1975
- Physical studies on DNA from "primitive" eucaryotes.1975
- AUTOMATED ANALYSIS OF DEOXYRIBONUCLEIC ACID, PROTEIN AND NUCLEAR TO CYTOPLASMIC RELATIONSHIPS IN TUMOR CELLS AND GYNECOLOGIC SPECIMENSJournal of Histochemistry & Cytochemistry, 1974
- Fluorescence correlation spectroscopy. II. An experimental realizationBiopolymers, 1974
- Fluctuation Spectroscopy: Determination of Chemical Reaction Kinetics from the Frequency Spectrum of FluctuationsProceedings of the National Academy of Sciences, 1973
- Chromosome-sized DNA molecules from DrosophilaChromosoma, 1973
- Molecular weights of coliphages and coliphage DNAJournal of Molecular Biology, 1970
- Complex formation between ethidium bromide and nucleic acidsJournal of Molecular Biology, 1965
- Viscosity and sedimentation of the DNA from bacteriophages T2 and T7 and the relation to molecular weightJournal of Molecular Biology, 1965
- THE MOLECULAR WEIGHTS OF T2 BACTERIOPHAGE DNA AND ITS FIRST AND SECOND BREAKAGE PRODUCTSProceedings of the National Academy of Sciences, 1961