Initiation of adenovirus DNA replication in vitro requires a specific DNA sequence.
Open Access
- 1 November 1983
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 80 (21), 6446-6450
- https://doi.org/10.1073/pnas.80.21.6446
Abstract
Initiation of adenovirus DNA replication in vitro occurs on a linearized plasmid DNA containing 3327 base pairs of the adenovirus terminal sequence. Various deletions have been constructed in the plasmid DNA and their template activities examined. Deletions from an internal restriction enzyme cleavage site that retain only 20 base pairs or more of the adenovirus terminal sequence support initiation and limited chain elongation; deletions that leave 14 base pairs or less of the terminal sequence do not. All deletions extending from the very terminus of the adenovirus DNA destroy the template activity. The terminal 20 base pairs of adenovirus DNA contain a sequence A-T-A-A-T-A-T-A-C-C, which is perfectly conserved in the DNA from different serotypes of human adenovirus. Base changes within the conserved sequence greatly reduce the template activity. These results suggest that the terminal 20 base pairs constitute a functional origin for the initiation of adenovirus DNA replication in vitro.Keywords
This publication has 49 references indexed in Scilit:
- Purification of an adenovirus-coded DNA polymerase that is required for initiation of DNA replicationCell, 1982
- A common sequence in the inverted terminal repetitions of human and avian adenovimsesGene, 1982
- Identification of the gene and mRNA for the adenovirus terminal protein precursorCell, 1981
- Structure and gene organization in the transforming Hind III-G fragment of Ad12Cell, 1980
- Cold-sensitive regulatory mutants of simian virus 40Journal of Molecular Biology, 1980
- Regulatory mutants of simian virus 40: Constructed mutants with base substitutions at the origin of DNA replicationJournal of Molecular Biology, 1979
- Nucleotide sequence at the inverted terminal repetition of adenovirus type 2 DNABiochemical and Biophysical Research Communications, 1979
- The nucleotide sequences at the termini of adenovirus-2 DNAJournal of Molecular Biology, 1979
- Adenovirus DNA: Structure and function of a novel repliconCell, 1978
- Construction of a viable SV40 variant containing two functional origins of DNA replicationCell, 1978