Developmental maps of acetylcholinesterase and G4-antigen of the early chicken brain: Long-distance tracts originate from AChE-producing cell bodies

Abstract

After approaching the outer surface of the neuroepithelium, postmitotic cell bodies abruptly start to synthesize acetylcholinesterase (AChE). Their easy histochemical detection allows us to trace sensitively spationtemporal patterns of differentiation processes of the chicken nervous system. To investigate the relationship between postmitotic AChE production and the first formation of neurites, AChE histochemistry is combined here with immunohistochemistry using the neurite‐specific G4‐antibody. Spatial computer reconstructions from double‐stained serial sections of whole brains of H.H. stages 10–20 demonstrate that G4‐neurite expression spatiotemporally follows the expression of AChE in its complex polycentric pattern closely, the details of which have been described earlier. By comparing both differentiative steps at the single cell level reveals that a great majority (if not all) of the G4‐positive neurites originate from AChE‐positive cell bodies. Based on both the computer reconstructions as well as single cell analysis, including [³H]‐thymidine pulse‐experiments followed by autoradiography, we conclude, that AChE expression precedes formation of G4‐neurites by about 15 h. In addition, the reconstructions provide the first detailed maps of G4‐fiber tract formation and shows that G4‐neurites form fascicles, most of which travel over long distances to targets within or without the central nervous system (CNS). This is the first demonstration for the entire young chicken brain which verifies that AChE‐expressing cells, generally, are those that will establish efferents to distant targets.