A Biosensor Array Based on Polyaniline

Abstract

This paper describes the fabrication of polyaniline-based microsensors and microsensor arrays for the estimation of glucose, urea, and triglycerides. Microelectronics technology has been used to produce gold interdigitated microelectrodes on oxidized silicon wafers. Polymer deposition and enzyme immobilization has been done electrochemically. Electrochemical potential control has been used to direct enzyme immobilization to the chosen microelectrodes and prevent it at other microelectrodes in contact with the enzyme solution. This has enabled the immobilization of three different enzymes on three closely spaced microelectrodes, resulting in a sensor array which can analyze a sample containing a mixture of glucose, urea, and triolein in a single measurement using a few microliters of the sample. This strategy is quite general and can be extended to other enzyme−substrate systems to eventually produce an “electronic tongue”.