QUENCHING OF SINGLET OXYGEN BY HUMAN PLASMA
- 1 March 1990
- journal article
- research article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 51 (3), 299-303
- https://doi.org/10.1111/j.1751-1097.1990.tb01714.x
Abstract
— Direct measurements of the decay of singlet oxygen phosphorescence at 1270 nm were made in human plasma diluted with various amounts of deuterium oxide. The Stern-Volmer plot of the singlet oxygen lifetimes was linear up to 15% plasma concentration (vol/vol). Extrapolation of these measurements to 100% plasma content gave a singlet oxygen lifetime of 1.04 ± 0.03 JJLS in human plasma. Biological molecules accounted for 77% of the total singlet oxygen quenching while water accounted for 23% of the quenching. The contributions of various types of biological molecules to the total singlet oxygen quenching were calculated from their plasma concentrations and their quenching constants. Plasma proteins quenched most of the singlet oxygen. Uric acid also quenched a significant amount of singlet oxygen (12%). Tocopherols, carotenoids, ascorbic acid and bilirubin made only small contributions to the total singlet oxygen quenching (=s 4%).This publication has 20 references indexed in Scilit:
- PRODUCTS AND RELATIVE REACTION RATES OF THE OXIDATION OF TOCOPHEROLS WITH SINGLET MOLECULAR OXYGENPhotochemistry and Photobiology, 1988
- SOME PREVALENT BIOMOLECULES AS DEFENSES AGAINST SINGLET OXYGEN DAMAGEPhotochemistry and Photobiology, 1988
- Optical determination of the collisional lifetime of singlet molecular oxygen [O2(1.DELTA.g)] in acetone and deuterated acetoneJournal of the American Chemical Society, 1982
- Lifetime of singlet oxygen in solution directly determined by laser spectroscopyJournal of the American Chemical Society, 1982
- Chemistry of singlet oxygen. 36. Singlet molecular oxygen (1.DELTA.g) luminescence in solution following pulsed laser excitation. Solvent deuterium isotope effects on the lifetime of singlet oxygenJournal of the American Chemical Society, 1982
- Uric acid provides an antioxidant defense in humans against oxidant- and radical-caused aging and cancer: a hypothesis.Proceedings of the National Academy of Sciences, 1981
- CELLULAR AND SUBCELLULAR MECHANISMS OF PHOTODYNAMIC ACTION: THE 1O2 HYPOTHESIS AS A DRIVING FORCE IN RECENT RESEARCHPhotochemistry and Photobiology, 1978
- THE QUENCHING OF SINGLET OXYGEN BY AMINO ACIDS AND PROTEINSPhotochemistry and Photobiology, 1975
- ON THE MECHANISM OF QUENCHING OF SINGLET OXYGEN IN SOLUTIONPhotochemistry and Photobiology, 1973
- Solvent Deuterium Isotope Effects on Acid-Base EquilibriaJournal of the American Chemical Society, 1964