Cross-linking of endothelin 1 and endothelin 3 to rat brain membranes: identification of the putative receptor(s)
- 10 July 1990
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 29 (27), 6415-6418
- https://doi.org/10.1021/bi00479a012
Abstract
Affinity-labeling experiments with 125I-endothelin derivatives using bifunctional cross-linking reagents were carried out in an attempt to identify the polypeptide component(s) of the endothelin/sarafotoxin receptors in rat brain tissues. In rat cerebellum, cortex, and caudate putamen, endothelin 1 specifically labeled a major component with a molecular mass of around 53 000. In the same tissues endothelin 3 specifically labeled, in addition to the 53 000 band, a band of molecular mass of 38 000. This result clearly indicates that in the brain the endothelin binding site resides within a polypeptide of apparent Mr = 53 000. The possible presence of receptor subtypes is discussed with reference also to the reported identification of endothelin receptors in chick cardiac membrane and in rat mesangial cells.This publication has 3 references indexed in Scilit:
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- Autoradiographic visualization of the binding sites for [125I]endothelin in rat and human brainNeuroscience Letters, 1989
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