Specific Determination of Proline in Biological Materials.
- 1 October 1956
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 93 (1), 54-57
- https://doi.org/10.3181/00379727-93-22661
Abstract
A specific method for proline is reported, based on treatment of isatin-developed paper chromatograms with dilute acid. None of the 20 other amino acids of synthetic tissue culture medium M 150, including hydroxyproline, reacted by this procedure. The proline chromogen exhibits an absorption maximum at 600 m[mu]. The color is remarkably stable and can be used for the quantitative determination of proline over the range of 1 to 16 [mu]g.Keywords
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