Photo‐CIDNP study of the interaction between lac repressor headpiece and lac operator DNA
- 19 October 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 239 (1), 99-104
- https://doi.org/10.1016/0014-5793(88)80553-2
Abstract
Lac repressor headpiece (HP) and intact lac repressor have been studied using the photo‐CIDNP method. At neutral pH histidine 29, tyrosines 7, 12 and 17 and methionine 1 are polarised. His‐29 polarisations are weaker and broader in HP59 than in HP51 indicating that the C‐terminal octapeptide in HP59 adopts a conformation that allows an interaction with His‐29. The photo‐CIDNP spectra of intact lac repressor and HP51 are very similar, showing that the same residues are accessible to the photo‐excited flavin. An equimolar mixture of HP51 and a 14 base pair lac operator fragment strongly suppresses the photo‐CIDNP effect of tyrosines 7 and 17 and abolishes the His‐29 polarisations. The results are compared with earlier photo‐CIDNP measurements on a complex of headpiece with poly[d(AT)] and with a model derived from a 2D NMR study on a lac headpiece‐operator complex.Keywords
This publication has 17 references indexed in Scilit:
- Complex of lac repressor headpiece with a 14 base-pair lac operator fragment studied by two-dimensional nuclear magnetic resonanceJournal of Molecular Biology, 1987
- Determination of protein structures from nuclear magnetic resonance data using a restrained molecular dynamics approach: The lac repressor DNA binding domainBiochimie, 1985
- A protein structure from nuclear magnetic resonance data: lac Repressor headpieceJournal of Molecular Biology, 1985
- PROTEIN-DNA RECOGNITIONAnnual Review of Biochemistry, 1984
- lac Repressor headpiece binds specifically to half of the lac operator: a proton nuclear magnetic resonance studyBiochemistry, 1983
- Photo-CIDNP Studies of ProteinsPublished by Springer Nature ,1982
- Photochemically induced dynamic nuclear polarization investigation of complex formation of the NH2-terminal DNA-binding domain of lac repressor with poly[d(AT)].Proceedings of the National Academy of Sciences, 1980
- DNA-binding site of lac repressor probed by dimethylsulfate methylation of lac operatorJournal of Molecular Biology, 1979
- An amino-terminal fragment of lac repressor binds specifically to lac operatorProceedings of the National Academy of Sciences, 1978
- Isolation of the amino-terminal fragment of lactose repressor necessary for DNA bindingBiochemistry, 1977