Quantitative assessment of Na+,K+-ATPase localization by direct and indirect p-nitrophenyl phosphatase methods.
Open Access
- 1 April 1987
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 35 (4), 507-513
- https://doi.org/10.1177/35.4.3029215
Abstract
Na+,K+-ATPase histochemistry, using direct (Pb) and indirect (Mg/Sr-Co) p-nitrophenyl phosphatase methods, was assessed by scanning integrative microdensitometry of three classes of tubules in mouse and guinea pig kidneys. The methods yielded similar and appropriate patterns of activity distribution and inhibitor response. The indirect method gave preferable results, in that non-enzymic background was lower and rate of reaction product accumulation was considerably higher.This publication has 8 references indexed in Scilit:
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