Stereochemical Studies of Hydrogen Incorporation from Nucleotides with Fatty Acid Synthetase from Brevibacterium ammoniagenes1
- 1 April 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 81 (4), 1167-1173
- https://doi.org/10.1093/oxfordjournals.jbchem.a131543
Abstract
The biosynthesis of fatty acids from malonyl-CoA and acetyl-CoA was investigated with an enzyme preparation which was purified 100-fold from Brevibacterium ammoniagenes. Fatty acids synthesized in the presence of D2O and stereospecifically deuterated NADPH and NADH were isolated and analyzed by mass chromatography to examine the localization of deuterium in the molecule. The following results were obtained:Keywords
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- Exchange of Hydrogen between Reduced Diphosphopyridine Nucleotide and WaterJournal of Biological Chemistry, 1959