Quantitative estimation of nicotinic acid in biological material

Abstract

The colorimetric method involving the use of cyanogen bromide and metol for the estimation of nicotinic acid (descr. previously) is fairly specific, and the technique involving alkaline hydrolysis and subsequent extraction of the nicotinic acid by means of acetone for analyzing biological material and medical organic preps. is descr. Of the different animal tissues the liver has the highest content of nicotinic acid, about 12 mg/100 g. 48 mg of nicotinic acid in 100 g are found in yeast; 15 mg/100 g in stomach; and from 2 to 122 mg/100 g in liver preps.