Nuclear magnetic resonance studies on transfer ribonucleic acid: assignment of AU tertiary resonances
- 4 September 1979
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 18 (18), 4005-4011
- https://doi.org/10.1021/bi00585a025
Abstract
The H--bonded ring NH NMR spectra of several tRNA species were examined with particular emphasis on the extreme low-field portion. Between -13.8 and -15 ppm there are 2 extra resonances which are not derived from cloverleaf base pairs. A combined approach involving undermodified tRNA, chemical modification and hairpin fragment studies assigned the T54-A58 resonance at -14.3 ppm in yeast tRNAPhe and Escherichia coli tRNA1Val; the U8-A14 resonance was assigned at -14.3 ppm and the s4U8-A14 resonance in bacterial tRNA was assigned at -14.9 ppm. The T54-A58 resonance shifts between -14.3 and -13.8 ppm depending on the surrounding nucleotide sequence in the ribothymidine loop.This publication has 10 references indexed in Scilit:
- Paramagnetic ion effects on the nuclear magnetic resonance spectrum of transfer ribonucleic acid: assignment of the 15-48 tertiary resonanceBiochemistry, 1979
- Identification of tertiary base pair resonances in the nuclear magnetic resonance spectra of transfer ribonucleic acidBiochemistry, 1979
- High-resolution nuclear magnetic resonance determination of transfer RNA tertiary base pairs in solution. 1. Species containing a small variable loopBiochemistry, 1977
- Crystallographic refinement of yeast phenylalanine transfer RNA at 2·5Å resolutionJournal of Molecular Biology, 1976
- Structural Domains of Transfer RNA MoleculesScience, 1976
- Quantitative determination of the number of secondary and tertiary structure base pairs in transfer RNA in solutionBiochemistry, 1976
- Changes in tertiary structure accompanying a single base change in transfer RNA. Proton magnetic resonance and aminoacylation studies of Escherichia coli tRNAMetf1 and tRNAMetf3 and their spin-labeled (S4U8) derivativesBiochemistry, 1976
- Similarity of the crystal and solution structure of yeast tRNAPheNature, 1976
- Three-Dimensional Structure of a Transfer RNA in Two Crystal FormsScience, 1976
- The Separation of Soluble Ribonucleic Acids on Benzoylated Diethylaminoethylcellulose*Biochemistry, 1967