Malate Dehydrogenase: Multiple Forms in Separated Blastomeres of Sea Urchin Embryos
- 18 October 1963
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 142 (3590), 389-390
- https://doi.org/10.1126/science.142.3590.389
Abstract
Sea urchin embryos at the 64-cell stage were dissociated by treatment with trypsin and separated by centrifugation on a sucrose gradient. The large blastomeres have two and the small blastomeres have three bands of L-malate dehydrogenase activity, which are separated by disk microelectrophoresis on polyacrylamide gel, whereas unfertilized eggs have five.Keywords
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