Volume-regulatory K+ efflux during concentrative uptake of alanine in isolated rat hepatocytes
- 1 July 1984
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 221 (1), 265-268
- https://doi.org/10.1042/bj2210265
Abstract
Changes in cell volume and 42K+ efflux associated with concentrative alanine uptake were studied in isolated rat hepatocytes suspended in Krebs-Ringer bicarbonate buffer. After addition of 10 mM-alanine, cellular water volume increased by 15% and the rate constant of 42K+ efflux by 250%. Alanine-induced 42K+ efflux was abolished by quinine and was strongly decreased when the cell-volume increase was counteracted by sucrose. The results suggest that K+ efflux during alanine uptake is implicated in a volume-regulatory response.This publication has 16 references indexed in Scilit:
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