Synthetic oligonucleotide tails inhibitin vitroandin vivotranslation of SP6 transcripts of maize zein cDNA clones
- 1 January 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 14 (3), 1511-1524
- https://doi.org/10.1093/nar/14.3.1511
Abstract
A maize zein cDNA clone was used to synthesize mRNA with the SP6 in vitro transcription system. Although we obtained full-length transcripts of the cDNA sequence, these were inefficient templates for protein synthesis. Removal of the 5' oligo(G) sequence that was synthesized during the cDNA cloning procedure allowed efficient translation of the mRNAs in a wheat germ cell-free protein synthesis system or in Xenopus laevis oocytes. The alteration in translational efficiency did not result from an interaction of the 5' oligo(G) homopolymer tail with the 3' oligo(C) sequence, as transcripts with or without the oligo(C) tail were translated similarly in both protein synthesis systems. Ribosome interaction with the mRNA may be affected due either to the secondary structure of the oligo(G) sequence itself, or an unusual secondary structure between the oligo(G) sequence and another region in the mRNA. Synthetic oligonucleotides at the 5' end of cloned cDNA sequences may generally be inhibitory for translation of mRNAs transcribed in vitro.Keywords
This publication has 19 references indexed in Scilit:
- Insertion mutagenesis to increase secondary structure within the 5′ noncoding region of a eukaryotic mRNA reduces translational efficiencyCell, 1985
- PARTIAL-PURIFICATION AND PROPERTIES OF A PRE-MESSENGER RNA SPLICING ACTIVITY1985
- In vitro-synthesized adenovirus 2 messenger RNA precursors are accurately spliced by nuclear extracts.Proceedings of the National Academy of Sciences, 1984
- Functional messenger RNAs are produced by SP6in vitrotranscription of cloned cDNAsNucleic Acids Research, 1984
- Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoterNucleic Acids Research, 1984
- Human β-globin pre-mRNA synthesized in vitro is accurately spliced in xenopus oocyte nucleiCell, 1983
- Synthesis and Deposition of Zein in Protein Bodies of Maize EndospermPlant Physiology, 1978
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- Synthesis of mitochondrial RNA by full-grown and maturing oocytes of Rana pipiens and Xenopus laevisDevelopmental Biology, 1975
- Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animalsJournal of Morphology, 1972