Serological identification and cellular distribution of three F9 antigen components.

Abstract

Using an affinity chromatography technique, IgM, IgG1 and IgG2a,b anti-F9 antibodies were isolated from the anti-F9 [mouse] serum; their activities were analyzed by immunofluorescence test on a variety of cell types, teratocarcinoma-derived cell lines and embryos. The anti-F9 antibodies react with at least 3 independent antigenic determinants not expressed on the same cell types, and that appear along different time-course during embryonic development.