Single-Molecule Detection by Laser-Induced Fluorescence Technique with a Position-Sensitive Photon-Counting Apparatus
- 1 March 1994
- journal article
- Published by IOP Publishing in Japanese Journal of Applied Physics
- Vol. 33 (3R), 1571-1576
- https://doi.org/10.1143/jjap.33.1571
Abstract
A new method of detecting single-molecule fluorescence has been implemented using a conventional optical microscope and a position-sensitive photon-counting apparatus. The samples were solvent-free fluorescent dyes adsorbed on a solid substrate in air at room temperature by spraying microdroplets of a dye solution followed by solvent evaporation. At the concentration used, each droplet contained a very small number of dye molecules. Quantized fluorescence intensity was determined and the number of occurrences of the quantized intensity agreed well with that calculated from Poisson's formula, thus confirming success of the position-sensitive single-molecule fluorescence detection.Keywords
This publication has 26 references indexed in Scilit:
- Magnetic resonance of a single molecular spinNature, 1993
- Change in Membrane Fluidity of Sand Dollar Egg Cortices Caused by Ca2+‐Induced Exocytosis: Microscopic Analysis with Fluorescence AnisotropyDevelopment, Growth & Differentiation, 1991
- A novel method for detection and counting of single bacteria in a wide field using an ultra-high-sensitivity TV camera without a microscopeFEMS Microbiology Letters, 1991
- Single-molecule detection of Rhodamine 6G in ethanolic solutions using continuous wave laser excitationAnalytical Chemistry, 1991
- Fluorescence spectroscopy and spectral diffusion of single impurity molecules in a crystalNature, 1991
- Excitation transport and fluorescence anisotropy of Rhodamine 3B on amorphous quartzThe Journal of Physical Chemistry, 1986
- Spectroscopy of Molecular Monolayers by Resonant Second-Harmonic GenerationPhysical Review Letters, 1982
- Primary Processes in the Photobleaching of Eosin as Revealed by the Flash TechniqueBulletin of the Chemical Society of Japan, 1960
- Irreversible Photobleaching of the Solution of Fluorescent Dyes. II. Photobleaching of the Alcoholic Solution of Eosine in VacuoBulletin of the Chemical Society of Japan, 1956
- Irreversible Photobleaching of the Solution of Fluorescent Dyes. III. Photoreaction of Eosine in the Aqueous Alcoholic Solutions under Evacuated StateBulletin of the Chemical Society of Japan, 1956