Peroxidase isozymes in wheat infected withTilletia controversa Kühn and rye infected withPuccinia recondita Rob. exDesm
- 1 July 1966
- journal article
- Published by Institute of Experimental Botany in Biologia plantarum
- Vol. 8 (4), 277-280
- https://doi.org/10.1007/bf02930662
Abstract
Peroxidase isozymes were studied in the case of two diseases:Tilletia controversa in wheat andPuccinia recondita in rye. The method employed was that of disc-electrophoresis on acrylamid gel. In the case of systemic disease (Tilletia controversa) no change was found in the isozyme pattern until a concentration of pathogen took place in the ears that began to form. Proofs are given that the new isozyme in unmatured ears comes from the pathogen. In the case of rye infected withPuccinia recondita a new isozyme was found; the latter does not originate in the pathogen, its origin is being discussed. Metodou diskovej elektroforézy na akrylamidovom géli boli študované izozymy peroxidázy u dvoch chorôb obilnín uTilletia controversa na pšenici aPuccinia recondita na raži. V prípade systémového ochorenia (Tilletia controversa) nebola zistená zmena v obraze izozymov až kým nedošlo k nakopeniu patogéna vo vytvárajúcich sa klasoch. Dokazuje sa, že nový izozym v nedozretých klasoch pochádza z patogéna. U raže infikovanej sPuccinia recondita bol zistený nový izozym, ktorý nepochádza z patogéna a diskutuje sa o jeho pôvode. По методу дисквого электрофореза изучались изозимы пероксидазы при двух заболеваниях эерновых,Tilletia controversa на пшенице иPuccinia recondita на рзи. В случае системного заболевания—Tilletia controversa— необнаружено изменений картины изозимов до наконления патогена в образующихся колосьях. Приводится доказательство происхождения нового изозима в колосьях из патогена. У ржи инфицированнойPuccinia recondita обнаружен новый изозим непроисходянций из натогена. Его происхождение обсуждается в данной работе.Keywords
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