Rapid Removal of Acid from Glycol Methacrylate for Improved Histological Embedding
- 1 January 1979
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 54 (5), 241-243
- https://doi.org/10.3109/10520297909110678
Abstract
A method for the rapid and complete removal of methacrylic acid from commercial samples of glycol methacrylate is presented. It entails conversion of the acid to an insoluble N-acylurea by treatment with an equivalent amount of N,N''-dicyclohexylcarbodiimide. Sections of [rat skin] tissue embedded in polymer prepared from the purified monomer can be stained with basic dyes without simultaneously staining the polymer.This publication has 4 references indexed in Scilit:
- Science and Art in Preparing Tissues Embedded in Plastic for Light Microscopy, with Special Reference to Glycol Methacrylate, Glass Knives and Simple StainsStain Technology, 1976
- Plant Microtechnique: Some Principles and New MethodsAmerican Journal of Botany, 1968
- Embedding Media for 1–2 Micron Sectioning. 2. Hydroxyethyl Methacrylate Combined with 2-ButoxyethanolStain Technology, 1967
- The Chemistry of Carbodiimides.Chemical Reviews, 1953