Analysis of the molecular species of the chick oviduct progesterone receptor using isoelectric focusing
- 21 August 1979
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 18 (17), 3679-3685
- https://doi.org/10.1021/bi00584a007
Abstract
Conditions are described for the preparative isoelectric focusing in flat beds of Sephadex of the progesterone [P] receptor from the chick oviduct. The method allows the fractionation of the receptor into 2 molecular species, one focusing at pI [isoelectric point] 6 and the other at pI 7 with good purification and recovery. The pI 6 and pI 7 receptor species were purified 2- and 26-fold, respectively. The assaying of the focused fractions with the charcoal binding method provides an accurate identification and quantitation of the [3H]P receptor. The method is reproducible in recovery, quantitation, and resolution of the 2 receptor species. The receptor with an apparent pI of 6 sediments at .apprx. 4 S on linear sucrose gradients, while the receptor with an apparent pI of 7 sediments at .apprx. 3.5 S. On the basis of the sedimentation values and elution patterns from DEAE chromatography, the pI 6 component is equivalent to the B receptor species and the pI 7 component is equivalent to the A receptor species described previously.This publication has 5 references indexed in Scilit:
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