Preventing Photochemistry in Culture Media by Long-Pass Light Filters Alters Growth of Cultured Tissues

Abstract

Exposure to plant tissue [Arabidopsis thaliana, <ycopersicon esculentum] culture media to light from fluorescent bulbs changed the growth regulating properties of the media. The light caused nutrient medium-dependent photosensitized degradation of the phytohormone indole-3-acetic acid and other media components. Photochemical changes in culture media were caused by light from 290 to 450 nanometers and were prevented with a yellow long-pass filter. The use of appropriately filtered light when culturing plant material can eliminate unnecessary variability by stabilizing the culture media composition.