Effect of Insulin on Albumin Production and Incorporation of14C-leucine into Proteins in Isolated Parenchymal Liver Cells from Normal Rats.

Abstract

Parenchymal rat liver cells were isolated by a modification of the collagenase method of Quistorff, Bondesen and Grunnet. The cells secreted albumin into the medium and incorporated ¹⁴C-leucine both into cell proteins and proteins secreted into the medium. Albumin production measured from the immunologically determined increment in the incubation medium was 1.7 ± 0.2, μg albumin/min per g liver wet wt. This is about 30% of the rate of production in the perfused liver. Addition of insulin (10-⁶-10^-10 M) enhanced albumin production (50-17%), and incorporation of ¹⁴C-leucine both into albumin (50-8%), secreted proteins (40-9%) and cell proteins (20-8%). Insulin does not increase the production of albumin by depleting the cells. The effect of insulin on albumin production is compatible with an effect on the rate of synthesis as the specific activity of albumin is unaffected by addition of insulin.