ANTIBODY FORMATION AGAINST VIBRIO CHOLERAE BY MESENTERIC LYMPH NODE CELL TRANSFER

Abstract

The intravenous transfer of mesenteric lymph node cells from intragastrically immunized donors [rabbits] led to the appearance of agglutinins in the sera of recipients. Other lymph node cells from these donors did not impart such a response in the recipients. The serum agglutinin titers in recipients were dependent on the immune state of the donor where the better immunization of the donor gave a high and prolonged response in the recipients. The antibody synthesizing property could be conveyed to the recipients by the intravenous transfer of any of the lymph node cells and spleen tissue using intravenously immunized donors. However, in intragastrically immunized donors, it was only the mesenteric lymph node cells that could effectively transfer this factor. Thus, the sensitization of the regional lymph nodes is important in cell transfer mechanism. The antibody that appeared in recipients after the cell transfer was not due to the transfer of preformed antibody but was due to the active participation of the recipient in the antibody synthesis. The lymph node cells tested in vitro were negative for Vibrio cholerae agglutinins.