A Gene for Lindane + Cyclodiene Resistance in the Red Flour Beetle (Coleoptera: Tenebrionidae)

Abstract

A field-collected strain of Tribolium castaneum (Herbst) resistant to lindane was genetically purified to make it isogenic with a laboratory-susceptible strain. Cross resistance extended to the cyclodienes dieldrin and oxychlordane but not to methoxychlor, avermectin, malathion, or chlorpyrifos methyl. Back crossing revealed that resistance was monogenic. Linkage analysis and genetic mapping showed that the cyclodiene resistance gene ( lin-H ) is located on the far left end of the third linkage group. Karyotype analysis of a T (Y;3) trans location confirmed that this linkage group is located on the longest autosome. Lin-R is the second insecticide resistance gene to be successfully mapped in T. castaneum .