The accessibility of 5-methylcytosine to specific antibodies in double-stranded DNA of Xanthomonas phage XP12
- 1 October 1985
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 152 (1), 115-121
- https://doi.org/10.1111/j.1432-1033.1985.tb09170.x
Abstract
Antibodies specifically directed to 5‐methylcytidine were raised in rabbits and purified by affinity chromatography. The accessibility of 5‐methyldeoxycytidine (m5dCyd) to such antibodies was studied with DNAs from various origins. The reaction was followed by measuring the retention of radiolabelled DNA by antibodies on nitrocellulose filters, by immunoprecipitation, by gel filtration and was visualized with the electron microscope. Antibodies did not bind to Escherichia coli B DNA, which is deficient in m5dCyd. Denatured and native DNA from calf thymus, which contains m5dCyd as a minor nucleoside, was weakly retained on the filters whereas DNA extracted from Xanthomonas oryzae XP12 bacteriophage, which is rich in m5dCyd, was well recognized even in the native form.This publication has 53 references indexed in Scilit:
- Hypomethylation of ras oncogenes in primary human cancersBiochemical and Biophysical Research Communications, 1983
- 5-Azacytidine stimulates fetal hemoglobin synthesis in anemic baboons.Proceedings of the National Academy of Sciences, 1982
- 5-Methylcytosine in Eukaryotic DNAScience, 1981
- Methylation of reiterated sequences in mammalian DNAs Effects of the tissue type, age, malignancy and hormonal inductionBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1981
- DNA Methylation and Gene FunctionScience, 1980
- DNA methylation in the human γδβ-globin locus in erythroid and nonerythroid tissuesCell, 1980
- DNA methylation: organ specific variations in the methylation pattern within and around ovalbumin and other chicken genesNucleic Acids Research, 1979
- Direct detection of methylated cytosine in DNA by use of the restriction enzyme MsplNucleic Acids Research, 1979
- The content of 5-methylcytosine in animal DNA: The species and tissue specificityBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- Rare Bases in Animal DNANature, 1970