Growth inhibition of Botrytis cinerea by compounds interfering with polyamine metabolism
- 1 June 1990
- journal article
- research article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 136 (6), 985-992
- https://doi.org/10.1099/00221287-136-6-985
Abstract
Summary: Several inhibitors of ornithine and arginine decarboxylases reduced growth of the fungus Botrytis cinerea cultured on Czapek Dox agar. Of these, the most effective were difluoromethylornithine (DFMO), dehydromonofluoro-methylornithine, difluoromethylarginine and dehydromonofluoromethylarginine. The growth inhibition due to 1 mM-DFMO could be partially reversed with 1 nM-putrescine. Other compounds causing significant reversal of DFMO-mediated growth inhibition included diaminopentane (cadaverine), diaminoheptane, spermidine, 7,7-difluorospermidine, spermine, bis(2-aminoethyl)amine, 2-hydroxy-1,3-diaminopropane, monoacetylputrescine, butenediamine and aminoguanidine. Some compounds, which were relatively innocuous by themselves, increased growth inhibition due to DFMO. Notably effective compounds were methylacetylenicputrescine, aminooxyaminopropane, butynediamine, 2,2-difluoroputrescine, diacetylputrescine, methylglyoxal bis(guanylhydrazone), streptomycin, certain methylated amines, and cyclohexylamine and related compounds. Growth inhibition due to a homologous series of diguanidines [NH2C(=NH)NH(CH2) x NHC(=NH)NH2] was also tested. These were especially effective when x = 12, and when x = 5 or 6. In general, the results suggest that amino-acid-based inhibitors of ornithine decarboxylase have a greater permeability than amine-based inhibitors.This publication has 15 references indexed in Scilit:
- Studies of non-metabolizable polyamines that support growth of SV-3T3 cells depleted of natural polyamines by exposure to α-difluoromethylornithineBiochemical Journal, 1988
- 1-Aminooxy-3-aminopropane reversibly prevents the proliferation of cultured baby hamster kidney cells by interfering with polyamine synthesis.Journal of Biological Chemistry, 1988
- The Effects of Polyamines on the Growth and Development of the Wheat Bunt FungiBotanical Gazette, 1988
- POLYAMINE DEPLETION AND GROWTH-INHIBITION IN CANDIDA-ALBICANS AND CANDIDA-TROPICALIS BY ALPHA-DIFLUOROMETHYLORNITHINE AND CYCLOHEXYLAMINE1988
- The oxidative decarboxylation of ornithine by extracts of higher plantsPhytochemistry, 1988
- Putrescine derivatives as substrates of spermidine synthaseInternational Journal of Biochemistry, 1987
- Characterization of Trypanosoma brucei brucei S-adenosyl-l-methionine decarboxylase and its inhibition by Berenil, pentamidine and methylglyoxal bis(guanylhydrazone)Biochemical Journal, 1986
- The Effects of Some Polyamine Biosynthetic Inhibitors on Growth and Morphology of Phytopathogenic Fungi1Plant and Cell Physiology, 1985
- Intracellular putrescine and spermidine deprivation induces increased uptake of the natural polyamines and methylglyoxal bis(guanylhydrazone)Biochemical Journal, 1980
- Antagonism between Basic Compounds (Spermine, Streptomycin) and Basic Bactericidal SubstancesNature, 1948