DNA Electrochemical Biosensor for the Detection of Short DNA Sequences Related to the Human Immunodeficiency Virus
- 1 August 1996
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 68 (15), 2629-2634
- https://doi.org/10.1021/ac9602433
Abstract
An electrochemical biosensor for the detection of short DNA sequences related to the human immunodeficiency virus type 1 (HIV-1) is described. The sensor relies on the immobilization and hybridization of the 21- or 42-mer single-stranded oligonucleotide from the HIV-1 U5 long terminal repeat (LTR) sequence at carbon paste or strip electrodes. The extent of hybridization between the complementary sequences is determined by the enhancement of the chronopotentiometric peak of the Co(phen)33+ indicator. Numerous factors affecting the probe immobilization, target hybridization, and indicator binding reactions are optimized to maximize the sensitivity and speed the assay time. A detection limit of 4 × 10-9 M HIV-1 U5 LTR segment is reported following a 30 min hybridization. The hybridization biosensor format obviates the use of radioisotopes common in radioactive methods for the detection of HIV-1 DNA. We also report on the direct adsorptive chronopotentiometric stripping measurements of trace levels of various HIV-1 DNAs.Keywords
This publication has 14 references indexed in Scilit:
- Electrochecmical biosensors for DNA sequence detectionElectroanalysis, 1996
- Real-time detection and quantification of DNA hybridization by an optical biosensorAnalytical Chemistry, 1995
- Trace Measurements of RNA by Potentiometric Stripping Analysis at Carbon Paste ElectrodesAnalytical Chemistry, 1995
- Detection of HIV1 DNA in Biological Samples by an Homogeneous Assay: Fluorescence Measurement of Double-Stranded RNA Synthesized from Amplified DNAAnalytical Biochemistry, 1994
- Decentralized electrochemical monitoring of trace metals: from disposable strips to remote electrodes. Plenary lectureThe Analyst, 1994
- Adsorption, immobilization, and hybridization of DNA studied by the use of quartz crystal oscillatorsAnalytical Chemistry, 1993
- Diagnostics in the Year 2000Published by Springer Science and Business Media LLC ,1993
- A Simple Method for Improved Assay Demonstrates that HIV p24 Antigen Is Present as Immune Complexes in Most Sera from HIV-Infected IndividualsThe Journal of Infectious Diseases, 1990
- Nucleic acid structure and expression of the human AIDS/lymphadenopathy retrovirusNature, 1985
- Optically active co-ordination compounds. Part XXXII. Potassium (+)tris-[L-cysteinesulphinato(2–)-SN]cobaltate(III): a versatile agent for resolution of 3+ speciesJ. Chem. Soc., Dalton Trans., 1973