Identification of a RNA polymerase II initiation site in the long terminal repeat of Moloney murine leukemia viral DNA.
- 1 September 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (9), 5411-5415
- https://doi.org/10.1073/pnas.78.9.5411
Abstract
A soluble in vitro RNA polymerase II transcription system was used to define the site of initiation of Moloney murine leukemia viral RNA synthesis. Molecularly cloned integrated and unintegrated Moloney murine leukemia virus DNA were used as templates. The 5'' ends of in vitro transcripts and virion RNA of Moloney murine leukemia virus were compared by nuclease S1 protection experiments. Viral sequences upstream of the in vivo cap site are implicated in the transcription of viral RNA and the 5'' end of an in vitro transcript derived from an integrated Moloney murine leukemia virus clone evidently corresponds to the 5'' end of viral genomic RNA.This publication has 42 references indexed in Scilit:
- Identification of a functional promoter in the long terminal repeat of Rous sarcoma virusCell, 1980
- Promoter Sequences of Eukaryotic Protein-Coding GenesScience, 1980
- Nucleotide sequence of an avian sarcoma virus oncogene (src) and proposed amino acid sequence for gene productNature, 1980
- Sequence of retrovirus provirus resembles that of bacterial transposable elementsNature, 1980
- A defined subgenomic fragment of in vitro synthesized Moloney sarcoma virus DNA can induce cell transformation upon transfectionCell, 1979
- Characterization of the mRNAs for α-, β- and γ-actinCell, 1977
- In vitro synthesis of infectious DNA of murine leukaemia virusNature, 1977
- RNA metabolism of murine leukemia virus: Size analysis of nuclear pulse-labeled virus-specific RNACell, 1977
- The reverse transcriptaseBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1977
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975