Extensive sequence homology at the 3′-termini of the four RNAs of cucumber mosaic virus
- 1 January 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 7 (4), 825-838
- https://doi.org/10.1093/nar/7.4.825
Abstract
The sequences of 270 residues from the 3'-termini of the four RNAs of cucumber mosaic virus have been determined by copying the in vitro polyadenylated RNAs with reverse transcriptase using d(pT8G) as primer and the 2',3'-dideoxynucleoside 5'-triphosphates as specific chain terminators. The terminal sequences of RNAs 3 and 4 were identical; this was expected since hybridization data has shown that the sequence of RNA 4 was present at the 3'-end of RNA 3 (Gould and Symons (1978) Eur. J. Biochem. 91, 269-278). The first 138 residues of RNAs 1 and 2 were identical to those of RNAs 3 and 4 except for one residue in RNA 1 and three residues in RNA 2. From residue 139 to 270 from the 3'-terminus, RNAs 1 and 2 showed, relative to RNAs 3 and 4, a non-homologous region of 33 residues, a homologous region of 40 residues, a partially homologous region of 14 residues which probably extended to about residue 300. There were 11 residues different between RNAs 1 and 2.Keywords
This publication has 25 references indexed in Scilit:
- Structure-independent nucleotide sequence analysis.Proceedings of the National Academy of Sciences, 1979
- Nucleotide sequence near the 5′-terminal of cucumber mosaic virus RNA No. 5 segmentFEBS Letters, 1979
- Complete sequence of constant and 3′ noncoding regions of an immunoglobulin mRNA using the dideoxynucleotide method of RNA sequencingCell, 1978
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- Sequence of an oligonucleotide derived from the 3' end of each of the four brome mosaic viral RNAs.Proceedings of the National Academy of Sciences, 1977
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977
- End labeling of enzymatically decapped mRNANucleic Acids Research, 1977
- Aminoacylation of RNA from Several Viruses: Amino Acid Specificity and Differential Activity of Plant, Yeast and Bacterial SynthetasesJournal of General Virology, 1974
- Comparative studies on tomato aspermy and cucumber mosaic virusesVirology, 1974
- Cucumber mosaic virus contains a functionally divided genomeVirology, 1973