Valency of Antibody Binding to Enveloped Virus Particles as Determined by Surface Plasmon Resonance
Open Access
- 15 January 2003
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 77 (2), 1649-1652
- https://doi.org/10.1128/jvi.77.2.1649-1652.2003
Abstract
A simple method is described for determining the valency of binding of immunoglobulin G to immobilized influenza A virus. Where there is a free Fab arm (monovalent binding), a second virus particle is captured. This is detected by surface plasmon resonance. The methodology should be applicable to all enveloped and nonenveloped viruses.Keywords
This publication has 31 references indexed in Scilit:
- Two Influenza A Virus-Specific Fabs Neutralize by Inhibiting Virus Attachment to Target Cells, While Neutralization by Their IgGs Is Complex and Occurs Simultaneously through Fusion Inhibition and Attachment InhibitionVirology, 2000
- Flexibility of the Major Antigenic Loop of Foot-and-Mouth Disease Virus Bound to a Fab Fragment of a Neutralising Antibody: Structure and NeutralisationVirology, 1999
- Structure of the complex of an Fab fragment of a neutralizing antibody with foot-and-mouth disease virus: positioning of a highly mobile antigenic loopThe EMBO Journal, 1997
- Direct Imaging of Interactions between an Icosahedral Virus and Conjugate Fab Fragments by Cryoelectron Microscopy and X-Ray CrystallographyVirology, 1994
- Structure of a human rhinovirus-bivalently bound antibody complex: implications for viral neutralization and antibody flexibility.Proceedings of the National Academy of Sciences, 1993
- IntroductionPublished by Springer Nature ,1993
- Antibody: the flexible adaptor moleculeTrends in Biochemical Sciences, 1990
- Different virus-precipitating activities of neutralizing monoclonal antibodies that recognize distinct sites of poliovirus particlesArchiv für die gesamte Virusforschung, 1987
- Generation of antibody diversity in the immune response of BALB/c mice to influenza virus hemagglutinin. I. Significant variation in repertoire expression between individual miceThe Journal of Experimental Medicine, 1983
- The antigenic structure of the influenza virus A/PR/8/34 hemagglutinin (H1 subtype)Cell, 1982