Affinity Chromatography of Phenylalanine Hydroxylase
- 1 April 1978
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 85 (2), 357-363
- https://doi.org/10.1111/j.1432-1033.1978.tb12247.x
Abstract
Four independent methods established that the structure of a previously reported pteridine affinity adsorbent, 6,7-dimethyl-5,6,7,8-tetrahydropterin.sbd.CH-Sepharose, is 5(CH-Sepharosyl)-6,7-dimethyl-5,6,7,8-tetrahydroprotein. A novel reaction, the carbodiimide-promoted coupling of a carboxyl group to N-5 of a tetrahydropterin, is described. Two novel adsorbents, 5-formyl-tetrahydrofolate.sbd.AH-Sepharose and 5-methyl-tetrahydrofolate.sbd.AH-Sepharose, are described which may be useful not only in the study of phenylalanine hydroxylase (EC 1.14.16.1) but also in the study of folate-metabolizing enzymes.This publication has 17 references indexed in Scilit:
- Dihydroxanthopterinuria in phenylketonuria and lethal hyperphenylalaninemia patientsClinica Chimica Acta; International Journal of Clinical Chemistry, 1977
- Purification of inactive phenylalanine hydroxylase protein from liver in classical phenylketonuriaNature, 1976
- Phenylalanine Hydroxylase of Macaca irusEuropean Journal of Biochemistry, 1975
- A pteridine adsorbent for affinity chromatographyFEBS Letters, 1974
- Adsorbents for affinity chromatography. Use of N-hydroxysuccinimide esters of agaroseBiochemistry, 1972
- Folate antagonists covalently linked to carbohydrates: Synthesis, properties, and use in the purification of dihydrofolate reductasesArchives of Biochemistry and Biophysics, 1972
- Isolation of the folate‐binding protein from cow's milk by the use of affinity chromatographyFEBS Letters, 1972
- Purification of dihydrofolic reductase from chicken liver by affinity chromatographyBiochemical and Biophysical Research Communications, 1971
- Affinity chromatography of dihydrofolate reductaseBiochemical Journal, 1971
- 2-Amino-4-hydroxy-6-methyl-7,8-dihydropteridine as a Model for Dihydrofolate*Biochemistry, 1967