Trout Leukocytes: Growth in Oxygenated Cultures

Abstract
A reproducible method of culturing leukocytes from rainbow trout (Salmo gairdneri) has been found. Using an atmosphere of 100 percent oxygen, we stimulated peripheral blood leukocytes to divide at 19°C. Cultured cells were used for a karyological study; they had a modal chromosome number of 60 and a constant arm number of 104, a value previously reported for other tissues in these fish.