Analysis and Detection of Chlamydial DNA
- 1 December 1984
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 130 (12), 3159-3164
- https://doi.org/10.1099/00221287-130-12-3159
Abstract
Elementary bodies of lymphogranuloma venereum (LGV) strains of Chlamydia trachomatis contain, in addition to the genomic DNA, a 6.7 kilobase plasmid. The plasmid from serovar L2 (434-B) was cloned at the BamHI site of pB327 into Escherichia coli and a restriction cleavage map of this pLGV125 recombinant plasmid was determined. All 15 C. trachomatis serovars contained DNA sequences that hybridized with pLGV125. When total DNA from L2 elementary bodies was used as a probe in Southern blotting and spot hybridization, serovars L1, L2 and L3 exhibited significant homology. The detection level of homologous DNA was 100 pg and LGV DNA was detectable in infected cells when total L2 probe was used in the nucleic acid hybridization test. These DNA probes may be useful as investigative and diagnostic reagents for C. trachomatis.This publication has 1 reference indexed in Scilit: