An Appraisal of Tests for Native DNA Antibodies in Connective Tissue Diseases

Abstract

Antibody of native [n] DNA was measured by 5 techniques: the C. luciliae immunofluorescence (CL-IF) test; filter radioimmunoassays [RIA] using untreated human [oral epidermoid carcinoma] KB cell DNA, endonuclease-treated KB DNA or a synthetic polynucleotide (Poly dAT); and the Farr immunoprecipitation assay using KB DNA. The specificity and sensitivity of the CL-IF assay was similar to that of the filter RIA procedures using KB DNA. The CL-IF test showed an increased frequency of positive tests in patients with active disease and severe renal involvement. In patients with severe renal involvement, high titers of serum nDNA antibodies were measured by this procedure. A unique advantage of the CL-IF test was its ability to identify complement-fixing nDNA antibody. The presence of such antibody was correlated with high antibody titer and the presence of severe renal disease. The CL-IF assay is a simple and useful procedure for measurement of anti-nDNA.