The collagen‐binding site of type‐II units of bovine seminal fluid protein PDC‐109 and fibronectin
- 1 November 1990
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 193 (3), 801-806
- https://doi.org/10.1111/j.1432-1033.1990.tb19403.x
Abstract
A single type-II domain has been isolated by limited proteolysis of the collagen-binding bovine seminal fluid protein, PDC-109. The 45-residue fragment corresponding to the second type-II domain of the parent molecule was found to have retained affinity for immobilized collagen, indicating that this minidomain carries critical regions of the collagen-binding site. Studies on various fragments of fibronectin have also implicated the two type-II units of this molecule in collagen-binding. In the present work we have found that type-II domains of human fibronectin, expressed in Escherichia coli as beta-galactosidase fusion proteins, bind specifically to immobilized collagen.Keywords
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