A FRACTIONATION OF THE HISTONES OF GROUP F2A FROM CALF THYMUS
- 1 January 1965
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 94 (1), 127-130
- https://doi.org/10.1042/bj0940127
Abstract
The calf-thymus histone group F2a has been separated into two sub-fractions by stepwise precipitation with acetone from acid solution. Carboxymethyl-cellulose and dextran-gel column chromatography and a method involving dialysis against ethanol have also given the sub-fractions, but the acetone-hydrochloric acid method has proved to be the most practicable. The two subfractions differ significantly in their amino acid composition and in the pattern of peptides obtained by tryptic digestion. Both fractions have a very low content of N-terminal amino acids and contain acetyl groups.Keywords
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