Modifications in Bowie's Staining of pepsinogen granules

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Abstract
Modifications of Bowie's technic for staining the pepsinogen granules of the body chief cells in the gastric glands, and a method of preparing the stomach of small rodents (e.g. the rat) for histo-topographical studies are described. The modifications involve counter-staining with aqueous alum hematoxylin, the use of the neutral synthetic mounting medium Permount, and changes in the timing of fixation, staining and differentiation.