Characterization of some pneumococcal bacteriophages
- 1 August 1976
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 19 (2), 659-667
- https://doi.org/10.1128/jvi.19.2.659-667.1976
Abstract
The growth of pneumococcal phages at high cell and phage densities is enhanced strongly by the substitution of K for Na in the medium. Initial titers of 2 .times. 1010 to 4 .times. 1010 PFU[plaque-forming units]/ml are readily obtained and concentrated stocks are stable in a storage buffer described. The mechanism of the cation effect is obscure. Phages .omega.3 and .omega.8 each have linear double-stranded DNA of 33 .times. 106 daltons/particle, with an apparent guanine plus cytosine content of 47-49 mol%, as determined by buoyancy and melting temperature, but with an unusual absorbance spectrum. Efficiency of plating is high if sufficient time is allowed for a relatively slow adsorption, which differs several-fold in rate between the 2 phages. Morphologically, these and other pneumococcal phages are similar to coliphage lambda but with a longer tail and tail fiber. Upon UV inactivation, .omega.3 and .omega.8 have D37 [37% survival dose] values of 33 and 55 J/m2, respectively, and each shows multiplicity reactivation. A total of 13 ts mutants were isolated from the 2 phages, representing only 2 complementation groups; complementation and recombination occur between .omega.3 and .omega.8 mutants. Both phages provoke high-titer antisera with extensive cross-reactivity against a number of newly isolated pneumococcal phages.This publication has 18 references indexed in Scilit:
- Pneumococcal bacteriophagesVirology, 1975
- “Diplophage”: A bacteriophage of Diplococcus pneumoniaeVirology, 1975
- An ATP-dependent deoxyribonuclease from Diplococcus pneumoniae: I. Partial purification and some biochemical propertiesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1970
- Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purificationVirology, 1970
- Multiplicity Reactivation as a Test for Recombination FunctionScience, 1969
- Studies on the physiological defect in rII mutants of bacteriophage T4Journal of Molecular Biology, 1966
- Sedimentation studies of the size and shape of DNAJournal of Molecular Biology, 1965
- Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperatureJournal of Molecular Biology, 1962
- Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsClJournal of Molecular Biology, 1962
- Physiological effects of rII mutations in bacteriophage T4Virology, 1961