Integration in vivo into simian virus 40 DNA of a sequence that resembles a certain family of genomic interspersed repeated sequences.
- 1 August 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (8), 4514-4518
- https://doi.org/10.1073/pnas.77.8.4514
Abstract
The isolation and characterization of a viable mutant of SV40 called in1449 are described. The mutant DNA is found to have a 157-nucleotide-long insertion at map position 0.649 within the 5'' untranslated sequence of the early region of SV40. The complete nucleotide sequence of the insert is presented. Sequence comparisons show that the insert is not of SV40 origin. The insert is presumably of monkey origin since in1449 was produced within [African green] monkey kidney cells. The sequence of the in1449 insert matches remarkably well with sequences of a certain predominant family of interspersed repeated sequences in human DNA (called the Alu family) and cloned members thereof. This high degree of sequence homology suggests that the in1449 inset is derived from a member of a family of interspersed repeated sequences in monkey DNA related to the human Alu family. The in1449 insert (and the Alu family members) contain certain oligonucleotide sequences that also are found conserved in the replication origins of papovaviruses and certain other oligonucleotides found in repetitive double-stranded regions of mammalian heterogeneous nuclear RNA. Sequences around the 2 recombinant joints in in1449 exhibit a definite pattern of homology. An octanucleotide present in the SV40 part of the 1st recombinant joint is exactly repeated 15 nucleotides away within the insert; another octanucleotide present within the insert at the 2nd joint is exactly repeated 21 nucleotides away in the viral DNA. The viral DNA sequences flanking the insert in in1440 also exhibit some homology.This publication has 31 references indexed in Scilit:
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977
- NUCLEOTIDE-SEQUENCE OF A FRAGMENT OF SV40 DNA THAT CONTAINS ORIGIN OF DNA-REPLICATION AND SPECIFIES 5' ENDS OF EARLY AND LATE VIRAL-RNA .3. CONSTRUCTION OF TOTAL SEQUENCE OF ECORII-G FRAGMENT OF SV40 DNA1977
- The Genome of Simian Virus 40Published by Elsevier ,1977
- The arrangement of simian virus 40 sequences in the DNA of transformed cellsCell, 1976
- Construction and analysis of viable deletion mutants of simian virus 40Journal of Virology, 1976
- Integrated simian virus 40 sequences in transformed cell DNA: analysis using restriction endonucleases.Proceedings of the National Academy of Sciences, 1976
- Evolutionary variants of simian virus 40: Cloned substituted variants containing multiple initiation sites for DNA replicationVirology, 1975
- The integrated state of viral DNA in SV40-transformed cells.Proceedings of the National Academy of Sciences, 1968
- Selective extraction of polyoma DNA from infected mouse cell culturesJournal of Molecular Biology, 1967
- A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.Proceedings of the National Academy of Sciences, 1967